A méhészeti termékek és a szintetikus antioxidáns hatásának összehasonlítása kísérleti szürkehályog esetén

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В.А.Колесников:

Antioxidant activity of bee products versus synthetic antioxidants at experimental cataract

Cataracts - the most common pathology of the eye, leading to a sharp decrease of view. The main role in its pathogenesis plays a rose-shenie activity of lipid peroxidation (LPO) of the lens and the reduction of antioxidant protection, which determines the desirability of studying the antioxidant agents to be included in the complex of conservative treatment of cataracts.

We have previously shown in the experiment was pronounced antioxidant-tion and the therapeutic effect of the synthetic antioxidant ionol with diquat-induced cataract.

The aim of this study is a comparative evaluation of en-tioksidantnoy activity of natural antioxidants - products pche-lovodstva and synthetic antioxidant - ionol model diquat-induced cataract.

Material and methods. Work performed on 63 rabbits breed Shin-Shilla weighing 2.0-2.5 kg in both eyes which was modeled oxidative-tive stress as a cause of the development of cortical cataract, a single intravitreal diquat dibromide.

In different series used to treat cataracts in-triple stillyatsii apiproduktov following formulation: 50% honey solution, a 1% solution of native royal jelly, propolis extract water (1: 5) apikompozitsii comprising all of these drugs, and 2 , a 2% solution of ionol. The effect of treatment was assessed by clinical cataract and biochemical changes show-teley lens.

The animals were taken out of the experience by gas embolism under anesthesia tiopen-tall by 14, 28, 42 and 56th day. From enucleated eyes you was isolated, lens, in which the tissue, the concentration of malonic-dialdehyde (MDA) to characterize the activity of processes of lipid peroxidation and activity of antioxidant enzymes.

Results. The clinical picture of cataract without treatment, characterized the call appearance of cataract, subcapsular and Corte-local vacuole, protein aggregates in the cortical layers of the lens, on-swelling of lens tissue disorganization and destruction of fibers co-ture of the lens.

When cataracts development there was a significant increase in the con-centration of MDA, the most pronounced on the 28th day, when it is 20 times exceeds the pre-norm. At the same time significantly reducing of the AOC-lens total capacity was recorded and the reduction of activity Gluten-thione-dependent antioxidant enzymes. Thus, the duration of the lag phase of MDA accumulation during the study period was knowledge, considerably lower than the norm and the 56th day experience was only 20% of the counter-la. The activity of glutathione-dependent enzymes AO-las significantly reducing, and glutathione reductase to the 56th day, it was lower by 86% of control. During all periods of observation is almost twice lower than normal concentration was restoration

Formation of glutathione. The concentration of the recovered protein Thio-mal groups to the 56th day decreased relative to the norm twice.

The greatest clinical effect was observed in the treatment of APIC-Plex and ionol when stabilization and regression of cataract have been fixed, and honey and propolis drugs caused only some stabilization cataract process.

The use of study drugs significantly reduced asset-ness POL, but the degree of reduction for different drugs was time-personal. The greatest concentration of MDA with respect to counter-la decreased by 14 th and 28 th day in the application ionol. Comparable with it results were obtained when assigning and apicomplexa natively of royal jelly.

When treating apikompozitsiey and ionol was an increase in the total capacity of the AOC, as well as a positive impact on the activity of Glu-tation-dependent antioxidant enzymes, which was Existing-tively higher in comparison with the animals without treatment, starting from the 14th day to the end of the experiment. glutathione peroxidase activity on the 56th day in the treatment of ionol greater than control at 336.6% and in the treatment position, APIC - 330.2% Activity on glutathione-SH-transferase (aGSH-tr) using Ionol was significantly higher than without treatment, all periods of observation and on the 56th day, it was an order of magnitude higher than the control (to 983.4%). In the treatment of all the results apikompozitsiey-Tats periods were comparable to ionol. Therapy ionol as apikompozi-tion, accompanied by a significantly higher activity glutati-onreduktazy (aGSSG-red) lens than without treatment. The concentration of Sun-Formation glutathione (kGSH) using ionol was Conoco-tavima with apikompozitsiey and 9-15 times higher than the control.

To a lesser extent it expressed a positive impact on all the above indicators of other bee products, especially honey and propolis.

The duration of the lag phase of MDA accumulation was the most high-Coy using ionol slightly less - apikompozitsii. By the 56th day in the treatment of these drugs lag phase duration was close to the level of standards.

Therapy study drug caused uneven An increase of duration-PAH-MDA. Treatment with ionol accompanied, was given a higher level of PAH-length MDA relative, but honey, propolis and royal jelly during all periods of observation, and in apikompozitsiey group exceeded this level only at 42 th day, the rest periods between the figures are not significantly different.

Conclusions

1. Application for diquat-induced cataract apiproduktov and synthetic antioxidant - ionol led to marked clinical effect, decrease the concentration of MDA and increase ak-efficiency antioxidant enzymes.

2. Among the tested bee products most pronounced en-tioksidantnym action comparable to ionol has apikompozitsiya.

3. The least pronounced antioxidant properties when studied-ener- pathology have honey and propolis